- Linux 64-bit environment (Ubuntu, CentOS, Fedora, Red hat) - To run the parsing scripts provided on this website.
- RAM - as required by mothur. (Development and validation of this database using mothur was performed on a server with 64 GB RAM)
- Around 2 GB Storage - for database, 8mer probability matrix and output files generated.
- tar - a LINUX software utility for unarchiving the downloaded database.
- mothur - a bioinformatics tool for analyzing 16S rRNA gene sequences. To download and setup mothur, you may follow the instructions as described here.
- D3 library - a JavaScript library for visualizing data with HTML, SVG, and CSS.
- CROP - a tool to cluster 16s rRNA sequences for OTU Prediction. This will assist in ‘open-reference OTU-picking’. Download CROP at this link
- CD-HIT - a widely used program for clustering sequences. This is an alternate to CROP for ‘open-reference OTU-picking’. Download CD-HIT at this link